Coding

Part:BBa_K2287024:Design

Designed by: Jiang Wu   Group: iGEM17_UCAS   (2017-10-20)


purD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 789
    Illegal AgeI site found at 1029
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Did no further optimization for expression in E.coli is needed.



Source

This sequence can directly obtained by PCR from genomic DNA of E.coli strain MG1655.

References